Halt Protease Inhibitor Cocktail （100X）,*，产品货号/编号：（Thermo）pierce-87786,规格/价格/报价 或在线咨询！
Halt Protease Inhibitor Cocktail
Stop proteolysis fast with a ready-to-use protease inhibitor cocktail.Thermo Scientific Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures.
Each 100X liquid cocktail contains optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide （DMSO）. The formulation provides nearly complete inhibition of the serine-proteases,cysteine-proteases, aspartic acid-proteases and aminopeptidases thatare typically present in cellular lysate samples. The identity and concentration of each component in the Halt Protease Inhibitor Cocktail is fully disclosed, leaving no uncertainties about variables and compatibility with experiments. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The stable, concentrated liquid cocktails are more effective and easier to use than traditional tablets. Available in several different package sizes, Halt Protease Inhibitor Cocktails are exceptionally convenient for either small-scale or large-scale needs.
Halt Protease Inhibitor Cocktails are easy to use. Simply add 10µL of concentrated cocktail per 1mL of lysis buffer to ensure complete protection of the resulting protein extract. Both EDTA and EDTA-free formulations of Halt Protease Inhibitor Cocktails are available in single-use format （24 x 100µL microtubes） and 1mL, 5mL and 10mL package sizes. The single-use format is ideal for small-scale cell lysis experiments, where each 100µL vial provides just what is needed for a 10mL sample and there is no risk of contaminating or degrading remaining aliquots of cocktail. Each microtube in the strip is heat-sealed with a foil that can be easily punctured by a pipette tip for trouble-free access to a precise amount of Halt Protease Inhibitor Cocktail needed for each experiment. Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases.Highlights of Halt Protease Inhibitor Cocktails:
Applications for Protease Inhibitor Cocktails:
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