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中文名称: 荧光素酶
其他中文名称: 荧光素酶(萤火虫);重组型酵素
荧光素酶英文名称: Luciferase firefly
其他英文名称: Luciferase from Photinus pyralis (firefly)
产品规格: BR,98%
包装: 1毫克
CAS号:61970-00-1
级别:BR
product line:BioUltra
Assay:≥98% (SDS-PAGE)
Mol wt:mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein:10-35% E1%/280
Measuring range:≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)
Foreign activity:ATPase ≤5 nanomolar units/mg protein;Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定义:One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
产品描述:Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximay 1.3 x 106 light units
性状:粉末。EC:1.13.12.7
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A67891 GRO human 人肿瘤生长相关因子 48T
A67892 HGH human 人生长激素 48T
A67893 HGF human 人肝生素 48T
A67894 HMGB1 human 人高迁移率族蛋白 48T
A67895 sICAM-1 human 人可溶性细胞间粘附分子-1 48T
A67896 sICAM-2 human 人可溶性细胞间粘附分子-2 48T
A67897 FGF-7 human 人角蛋白细胞生长因子 48T
A67898 FGF-9 human 人成纤维生长因子-9 48T
A67899 GLP-1(human) 人胰高血糖素样肽1 48T
A67900 TGF Beta 2 人转化生长因子 β 2 48T
A67901 TGF Beta 1 人转化生长因子 β 1 48T
A67902 ER(Estrpgen Receptor)Human 人雌激素受体 48T
A67903 PR(Progestogerone receptor)Human 人孕激素受体 48T
A67904 EGF 人表皮生长因子 48T
A67905 EGFR 人表皮生长因子受体 48T
A67906 ERK1 human 人丝裂原活化蛋白激酶1 48T
A67907 human-VEGF/ELISA 人血管内皮生长因子 48T
A67908 human-VEGF-D 人血管内皮生长因子D 48T
A67909 VEGF-R1 人血管内皮生长因子受体1 48T
A67910 VEGF-R2 人血管内皮生长因子受体2 48T
A67911 UPAR 人尿激酶样纤溶酶原活化物受体 48T
A67912 TH1/TH2 人细胞内细胞因子 48T
A67913 HLA-G 人类白细胞抗原G 48T
A67914 CTGF 人结缔组织生长因子 48T
A67915 NPY(humen) 人神经肽Y 48T
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A67918 MIP-1 Alpha 人巨噬细胞炎性蛋白-1α 48T
A67919 MIP-1 Gamma 人巨噬细胞炎性蛋白-1γ 48T
A67920 MIP-1 Beta 人巨噬细胞炎性蛋白-1β 48T